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Real-time RT-PCR assay for quantitative detection of BCR-ABL1 fusion gene, which results from translocations t(9;22), identifying two primary mRNA isoforms: p210, linked to Chronic Myeloid Leukemia (CML), and p190, associated with Philadelphia-positive Acute Lymphoblastic Leukemia (Ph+ ALL). As a reflex, qualitative RT-PCR for detection of t(9;22)transcripts that result in p230, can be ordered when BCR-ABL1 Standardp210, p190 is negative. These RNA-based tests help in diagnosis, monitoring treatment effectiveness, and detecting minimal residual disease(MRD).
Whole blood (3-5 mL), Bone marrow(1-2 mL), in Lavender-top (EDTA) tube.
Use cold pack for transport, making sure cold pack is not in direct contact with specimen. Ship same day as drawn whenever possible; specimens <7 days old preferred.