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Bi-directional sequencing analysis on KIT exons 8, 9, 11, 13 and 17 for detection of activating mutations including the common mutation D816V, which are found in gastrointestinal stromal tumors (GIST), melanoma, core-binding factor AML(CBF-AML), mast cell disease (systemic mastocytosis), and germ cell tumors.Mutation identification is useful for planning TKI therapy and predicting the clinical course.
Whole blood (5 mL), Bone marrow(2 mL), in Lavender-top (EDTA) tube.
Formalin-fixed, paraffin-embedded (FFPE) tissue block (preferred) or slides (1 H&E slide, plus 5-10 unstained slides cut at 5+ microns). Please use positively charged slides and 10% NBF fixative. Do not use zinc fixatives.
Use cold pack for transport.Make sure cold pack is not in direct contact with specimen.