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A cell surface glycoprotein expressed on all thymocytes, T cells, myeloid lineage cells, and most leukocytes except resting B cells. Useful in identifying T-cell lymphomas and a subset of B-cell lymphomas. Co-expression with CD20 occurs in some low-grade lymphomas, and expression is often correlated with CD5; follicular lymphoma is rarely positive.
Tissue Block: Formalin-fixed, paraffin-embedded (FFPE) tumor block preferred.
Unstained Slides: Recommended 2 charged slides per stain requested (minimum of 1 slide per stain), baked or unbaked.
Fixation: Tissue must be fixed in 10% neutral buffered formalin for 6–72 hours.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.