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This antibody cocktail is used to distinguish luminal (ER-positive) from basal-like (CK5-positive) breast carcinomas. Estrogen receptor (ER) identifies hormone-responsive luminal epithelial cells, while cytokeratin 5 (CK5) highlights basal/myoepithelial cells. The combined stain aids in subtyping breast cancers for prognosis and treatment planning, as well as differentiating benign proliferations from malignant lesions.
Tissue Block: Formalin-fixed, paraffin-embedded (FFPE) tumor block preferred.
Unstained Slides: Recommended 2 charged slides per stain requested (minimum of 1 slide per stain), baked or unbaked.
Fixation: Tissue must be fixed in 10% neutral buffered formalin for 6–72 hours.
Use cold pack for transport. Make sure cold pack is not indirect contact with specimen.