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Next-generation sequencing assa yto detect mutations on exons 12-15 of the JAK2 gene, intended as are flex for cases in which JAK2 V617F is not detected (ordered separately). JAK2 exon 12 mutated in polycythemia vera (PV) patients, shows similar prognosis to JAK2 V617F mutated cases. Other rare non-V617F mutations can be detected in exons 13, 14 and 15 of the JAK2 gene.
Whole blood (5 mL), Bone marrow(2 mL), in Lavender-top (EDTA) tube. Sodium Heparin is acceptable.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen. Do not freeze the sample.