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Detects cytokeratins 5 and 14, markers of basal and myoepithelial cells in stratified and complex epithelia. This cocktail is useful in identifying squamous differentiation, distinguishing basal-like breast carcinomas, and differentiating benign from malignant glandular lesions based on the presence or absence of basal/myoepithelial layers.
Tissue Block: Formalin-fixed, paraffin-embedded (FFPE) tumor block preferred.
Unstained Slides: Recommended 2 charged slides per stain requested (minimum of 1 slide per stain), baked or unbaked.
Fixation: Tissue must be fixed in 10% neutral buffered formalin for 6–72 hours.
Use cold pack for transport. Make sure cold pack is not indirect contact with specimen.