.png)
Bisulfite modification and Real-time PCR assays for quantitative analysis of the CpG methylation within the MGMT gene promoter, which has been shown in approximately 40% to 50% of glioblastomas and is associated with an increased likelihood of a favorable response to alkylating agents such as temozolomide (Temodar®).
Formalin-fixed, paraffin-embedded(FFPE) tissue block (preferred, 40% tumor or more) or slides (1 H&E slide,plus 5-10 unstained slides cut at 5+ microns. Please use positively-charged slides and 10% NBF fixative. Do not use zinc fixatives.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.