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Bisulfite modification and Real-time PCR assays to detect hypermethylation of the MLH1 gene promoter. It can help distinguish sporadic from inherited colorectal cancers. Percentage of methylated DNA (compared to total DNA) is reported for positive results.Analysis should be considered in combination with IHC, BRAF, and/or MSI.
Formalin-fixed, paraffin-embedded(FFPE) tissue block (preferred, 40% tumor or more) or slides (1 H&E slide,plus 5-10 unstained slides cut at 5 or more microns. Please use positively-charged slides and 10% NBF fixative. Do not use zinc fixatives.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.