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Identifies a nuclear protein expressed in germ cells of the testis and ovary. NUT midline carcinomas (NMCs) are highly aggressive tumors defined by chromosomal rearrangements involving the NUT gene on chromosome 15q14, leading to protein overexpression. Because NUT expression can also be seen in other tumors such as porocarcinoma, NUT IHC positivity alone is not diagnostic of NMC and should be confirmed with molecular testing.
Tissue Block: Formalin-fixed, paraffin-embedded (FFPE) tumor block preferred.
Unstained Slides: Recommended 2 charged slides per stain requested (minimum of 1 slide per stain), baked or unbaked.
Fixation: Tissue must be fixed in 10% neutral buffered formalin for 6–72 hours.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.