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Detects expression of tropomyosin receptor kinase A, B, and C proteins arising from the NTRK 1, 2, and 3 genes, respectively. NTRK gene rearrangements occur in many different tumor types, often at low frequency, including lung non-small cell carcinomas, colorectal and other GI-related adenocarcinomas, breast carcinomas, melanoma, renal cell carcinomas, and adult brain tumors. IHC screening is not recommended in neuroendocrine tumors, GISTs, gliomas, or adult sarcomas, as these tissues so positive staining in the absence of an NTRK translocation. Instead, molecular testing (i.e. NGS or FISH) for an NTRK gene fusion is recommended to confirm the presence of an NTRK gene fusion. Patients having a tumor harboring an NTRK gene fusion may be responsive to NTRK inhibitor therapy.
Tissue Block: Formalin-fixed, paraffin-embedded (FFPE) tumor block preferred.
Unstained Slides: Recommended 2 charged slides per stain requested (minimum of 1 slide per stain), baked or unbaked.
Fixation: Tissue must be fixed in 10% neutral buffered formalin for 6–72 hours.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.