Identifies programmed death-ligand 1 (PD-L1), a cell surface protein involved in immune evasion by tumor cells. PD-L1 expression helps assess eligibility for immune checkpoint inhibitor therapies and is seen in a variety of cancers, including lung, breast, bladder, and melanoma. The E1L3N clone is widely used for evaluating PD-L1 expression in tumor and immune cells within the tumor microenvironment.
Tissue Block: Formalin-fixed, paraffin-embedded (FFPE) tumor block preferred.
Unstained Slides: Recommended 2 charged slides per stain requested (minimum of 1 slide per stain), baked or unbaked.
Fixation: Tissue must be fixed in 10% neutral buffered formalin for 6–72 hours.
Use cold pack for transport. Make sure cold pack is not indirect contact with specimen.