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RNA-based next-generation sequencing targeting the transcriptome sequencing of more than 1600 genes to provide a comprehensive evaluation of cancer. The test is designed to detect microsatellite instability (MSI), tumor mutation burden (TMB), homologous recombination repair (HRR) mutations, and homologous recombination deficiency (HRD). Other notable features include evaluation for MET exon 14skipping, EGFRvIII, AR-V7, TERT promoter mutations, DYPD gene polymorphism for prediction of toxicity to fluoropyrimidine therapy, and RNA levels of CTLA4, PD-L1, and PD-L2. It can also detect gene expression levels that correlate to immunophenotype, gene amplifications, alternative splicing, HLA class I genotyping, viral infections (EBV, HPV, TTV), T-cell and B-cell Clonality and MGMT methylation.
Formalin-fixed, paraffin-embedded (FFPE): 1 H&E slide plus 6-8, at 5 or more microns fix with 10% NBF fixative.Please circle tumor for microdissection. Alternatively, the FFPE block can be sent for tumor circling and cutting at our laboratory.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.