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Identifies villin, an actin-binding protein present in human renal epithelial cells, used as a marker for gastrointestinal tract–derived carcinomas. Along with CDX2, villin is a highly specific and moderately sensitive marker for colorectal and other GI tract (pancreatobiliary) carcinomas, typically showing a brush border pattern. A subset of lung adenocarcinomas may also express villin.
Tissue Block: Formalin-fixed, paraffin-embedded (FFPE) tumor block preferred.
Unstained Slides: Recommended 2 charged slides per stain requested (minimum of 1 slide per stain), baked or unbaked.
Fixation: Tissue must be fixed in 10% neutral buffered formalin for 6–72 hours.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.